Is Ffh required for export of secretory proteins?

FEBS Letters

Kim, Jinoh¹  Luirink, Joen²  Rusch, Sharyn¹  Kendall, Debra A.¹ 

[1] Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269-3044, USA;Department of Microbiology, Institute of Molecular Biological Sciences, Biocentrum Amsterdam, 1081 HV Amsterdam, The Netherlands

关键词: phoA;    Protein export;    Signal recognition particle;    Secretory protein;    Trigger factor;    Escherichia coli;    Ffh;    fifty-four homolog;    MOPS;    morpholinepropanesulfonic acid;   

DOI  :  10.1016/S0014-5793(01)02784-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In Escherichia coli, protein export from the cytoplasm may occur via the signal recognition particle (SRP)-dependent pathway or the Sec-dependent pathway. Membrane proteins utilize the SRP-dependent route, whereas many secretory proteins use the cytoplasmic Sec machinery. To examine the possibility that signal peptide hydrophobicity governs which targeting route is utilized, we used a series of PhoA signal sequence mutants which vary only by incremental hydrophobicity changes. We show that depletion of SRP, but not trigger factor, affects all the mutants examined. These results suggest secretory proteins with a variety of signal sequences, as well as membrane proteins, require SRP for export.

【 授权许可】

Unknown