Involvement of a chloroplast homologue of the signal recognition particle receptor protein, FtsY, in protein targeting to thylakoids

FEBS Letters

Hirohashi, Toshiya¹  Nakai, Masato¹  Kikuchi, Shingo¹  Kogata, Naoko¹  Nishio, Kazuaki¹ 

[1] Institute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita, Osaka 565-0871, Japan

关键词: Signal recognition particle;    Thylakoid import;    FtsY;    Protein export;    Arabidopsis thaliana;    SRP;    signal recognition particle;    GTP;    guanosine 5′-triphosphate;    SDS-PAGE;    sodium dodecyl sulfate-polyacrylamide gel electrophoresis;    LHCP;    the light-harvesting chlorophyll a/b-binding protein;   

DOI  :  10.1016/S0014-5793(99)00305-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We isolated an Arabidopsis thaliana cDNA whose translated product shows sequence similarity to the FtsY, a bacterial homologue of SRP receptor protein. The Arabidopsis FtsY homologue contains a typical chloroplast transit peptide. The in vitro-synthesized 37 kDa FtsY homologue was imported into chloroplasts, and the processed 32 kDa polypeptide bound peripherally on the outer surface of thylakoids. Antibodies raised against the FtsY homologue also reacted with a thylakoid-bound 32 kDa protein. The antibodies inhibited the cpSRP-dependent insertion of the light-harvesting chlorophyll a/b-binding protein into thylakoid membranes suggesting that the chloroplast FtsY homologue is involved in the cpSRP-dependent protein targeting to the thylakoid membranes.

【 授权许可】

Unknown