FEBS Letters | |
The protonophore CCCP induces mitochondrial permeability transition without cytochrome c release in human osteosarcoma cells | |
Minamikawa, Tetsuhiro1  Nagley, Phillip1  Lim, Maria L.R1  | |
[1] Department of Biochemistry and Molecular Biology, P.O. Box 13D, Monash University, Clayton, Vic. 3800, Australia | |
关键词: Mitochondrial permeability transition; Cytochrome c; Bax; Cell death; Mitochondrion; | |
DOI : 10.1016/S0014-5793(01)02693-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Mitochondrial permeability transition (MPT) and cytochrome c redistribution from mitochondria are two events associated with apoptosis. We investigated whether an MPT event obligatorily leads to cytochrome c release in vivo. We have previously shown that treatment of human osteosarcoma cells with the protonophore m-chlorophenylhydrazone (CCCP) for 6 h induces MPT and mitochondrial swelling without significant cell death. Here we demonstrate that release of cytochrome c does not occur and the cells remain viable even after 72 h of treatment with CCCP. Bax is not mobilized to mitochondria under these conditions. However, subsequent exposure of CCCP-treated cells to etoposide or staurosporine for 48 h results in rapid cell death and cytochrome c release that is accompanied by Bax association with mitochondria, demonstrating competency of these mitochondria to release cytochrome c with additional triggers. Our findings suggest that MPT is not a sufficient condition, in itself, to effect cytochrome c release.
【 授权许可】
Unknown
【 预 览 】
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RO201912020310804ZK.pdf | 441KB | ![]() |