Sequence‐specific binding property of Arabidopsis thaliana telomeric DNA binding protein 1 (AtTBP1)

FEBS Letters

Hwang, Moo Gak¹  Kang, Bin Goo¹  Chung, In Kwon¹  Cho, Myeon Haeng¹ 

[1] Department of Biology, Yonsei University, Seoul 120-749, South Korea

关键词: Telomere-binding protein;    Telomere;    Electrophoresis mobility shift assay;    Arabidopsis thaliana;    AtTBP1;    Arabidopsis thaliana telomeric DNA-binding protein 1;    TBP;    telomere-binding protein;    SMTBD;    single Myb telomeric DNA-binding domain;    EMSA;    electrophoresis mobility shift assay;    GST;    glutathione S-transferase;    TAS;    telomere-associated sequences;   

DOI  :  10.1016/S0014-5793(01)02685-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have identified an Arabidopsis thaliana cDNA, designated as AtTBP1, encoding a protein with a predicted size of 70.6 kDa that specifically binds to the plant telomeric repeat sequence TTTAGGG. AtTBP1 is present as a single-copy gene in Arabidopsis genome and is expressed ubiquitously in various organs. AtTBP1 has a single Myb telomeric DNA binding domain at the C-terminus and an extensive homology with other known telomere-binding proteins. The isolated C-terminus of AtTBP1 is capable of sequence-specific DNA binding to plant duplex telomeric DNA. These results suggest that AtTBP1 may play important roles in plant telomere function in vivo.

【 授权许可】

Unknown