Inhibition of plant telomerase by telomere‐binding proteins from nuclei of telomerase‐negative tissues

FEBS Letters

Fajkus, Jiřı́¹  Fulnečková, Jana¹ 

[1] Department of Analysis of Biologically Important Molecular Complexes, Masaryk University Brno, Královopolská 135, CZ-61265 Brno, Czech Republic

关键词: Plant telomerase regulation;    Telomere-binding protein;    Telomerase inhibition;   

DOI  :  10.1016/S0014-5793(00)01178-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activity of telomerase in plant cells is precisely regulated in response to changes in cell division rate. To explore this regulatory mechanism, the effect on telomerase activity of protein extracts from nuclei of telomerase-negative tissues was examined. An inhibition of telomerase activity was found which was species-non-specific. This inhibition was due to proteins which form salt-stable, sequence-specific complexes with the G-rich telomeric strand and reduce its accessibility, as shown by gel retardation and by terminal transferase (TdT) extension of G-rich telomeric and non-telomeric (substrate) primers. A 40 kDa polypeptide was detected by SDS–PAGE after cross-linking the complex formed by extracts from tobacco leaf nuclei. Such proteins may be involved in regulation of telomerase activity in plants.

【 授权许可】

Unknown