Growth kinetics rather than stress accelerate telomere shortening in cultures of human diploid fibroblasts in oxidative stress‐induced premature senescence

FEBS Letters

Chainiaux, Florence¹  Dierick, Jean-François¹  Frippiat, Christophe¹  Royer, Véronique¹  Eliaers, François¹  Pascal, Thierry¹  Remacle, José¹  de Magalhaes, Joao Pedro¹  Dumont, Patrick¹  Toussaint, Olivier¹ 

[1] Department of Biology, Laboratory of Cellular Biochemistry and Biology, University of Namur (FUNDP), 61 Rue de Bruxelles, B-5000 Namur, Belgium

关键词: Telomere;    Aging;    Senescence;    Oxidative stress;    H2O2;    PD;    population doubling;    SIPS;    stress-induced premature senescence;    FCS;    fetal calf serum;    HDF;    human diploid fibroblast;    SA β-gal;    senescence-associated β-galactosidase;    t-BHP;    tert-butylhydroperoxide;    TRF;    terminal restriction fragment;   

DOI  :  10.1016/S0014-5793(01)02679-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

WI-38 human diploid fibroblasts underwent accelerated telomere shortening (490 bp/stress) and growth arrest after exposure to four subcytotoxic 100 μM tert-butylhydroperoxide (t-BHP) stresses, with a stress at every two population doublings (PD). After subcytotoxic 160 μM H₂O₂ stress or five repeated 30 μM t-BHP stresses along the same PD, respectively a 322±55 and 380±129 bp telomere shortening was observed only during the first PD after stress. The percentage of cells resuming proliferation after stress suggests this telomere shortening is due to the number of cell divisions accomplished to reach confluence during the first PD after stress.

【 授权许可】

Unknown