A maternal high fat diet represses the expression of antioxidant defense genes and induces the cellular senescence pathway in the liver of male offspring rats

Aging;Cancer;Oxidative stress;Non-alcoholic fatty liver disease;Lipid

Zhang, Xiyuan ; Pan ; Yuan-Xiang

关键词: Aging;    Cancer;    Oxidative stress;    Non-alcoholic fatty liver disease;    Lipid;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/24049/Zhang_Xiyuan.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

Maternal high fat (HF) diet feeding is associated with increased risk of developing metabolism-related diseases in adult offspring, including chronic liver disease. The present study tested the hypothesis that maternal high fat diet leads to a decreased antioxidant defense capacity and causes cellular senescence in liver of adult offspring rats, which might increase risk of developing chronic liver disease. Timed-pregnant Sprague Dawley rats were fed a HF diet (45% of energy from fat) or a control (C) diet (16% of energy from fat) during gestation and lactation. The resulting offspring were fed a control diet after weaning, to generate two offspring groups: C/C and HF/C. At 12 wk of age, male rats were killed and samples were collected for analysis. Maternal HF diet significantly increased plasma triacylglycerol and hepatic thiobarbituric acid reactive substance concentrations and the size of hepatic lipid droplets in offspring rats. The expression of antioxidant defense genes, such as glutathione peroxidase-1 (Gpx1), Cu/Zn superoxide dismutase (Sod1), paraoxonase enzymes (Pon1, Pon2, and Pon3) were significantly lower in the liver of HF/C pups than in C/C pups. The expression of p16INK4a, a marker of cellular senescence, and cyclooxygenase-2 (Cox2), a pro-inflammatory marker, was significantly higher in the HF/C offspring group than in the C/C offspring group. Western blot analysis shows that cyclin D1 and phosphorylated retinoblastoma (p-Rb) protein were significantly lower in HF/C offspring than in C/C offspring. The results provide the first evidence that maternal HF diet might alter antioxidant defense capacity and program the p16INK4a- dependent cellular senescence in the liver of adult offspring.