Apaf‐1 localization is modulated indirectly by Bcl‐2 expression

FEBS Letters

Albar, J.P¹  Martı́nez-A, Carlos¹  Ruiz-Vela, Antonio¹ 

[1] Department of Immunology and Oncology, Centro Nacional de Biotecnologı́a/CSIC, Universidad Autónoma de Madrid, Campus de Cantoblanco, E-28049 Madrid, Spain

关键词: B lymphocyte;    Apoptosis;    Subcellular localization;    Bcl-2;    Cytochrome c;    Apaf-1;    Apaf-1;    apoptotic protease activating factor-1;    β-COP;    β-coat protein;    FCS;    fetal calf serum;    PDI;    protein disulfide isomerase;   

DOI  :  10.1016/S0014-5793(01)02629-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Apoptotic protease activating factor-1 (Apaf-1) is an adaptor molecule essential for caspase-9 activation. Subcellular analysis of Apaf-1 in NIH-3T3 fibroblasts and the immature murine B cell lymphoma WEHI-231 indicates that Apaf-1 is localized in the Golgi apparatus and cytoplasm. Bcl-2 overexpression in WEHI-231 cells disrupts Apaf-1 localization in Golgi, causing a perinuclear Apaf-1 redistribution. Bcl-2 overexpression in NIH-3T3 fibroblasts however does not cause similar Apaf-1 redistribution, suggesting that cell type factors are involved in the redistribution process. The ability of Bcl-2 to modify Apaf-1 subcellular localization is not explained by direct interaction between Apaf-1 and Bcl-2. These data may help to clarify the anti-apoptotic Bcl-2 function.

【 授权许可】

Unknown