Transcriptome meets metabolome: hierarchical and metabolic regulation of the glycolytic pathway

FEBS Letters

Westerhoff, Hans V¹  ter Kuile, Benno H² 

[1] Molecular Cell Physiology and Mathematical Biochemistry, BioCentrum Amsterdam, De Boelelaan 1087, 1081 HV Amsterdam, The Netherlands;Leiden Institute of Chemistry, P.O. Box 9502, 2300 RA Leiden, The Netherlands

关键词: Metabolic control;    Hierarchical control;    Metabolic flux;    Regulation of transcription;   

DOI  :  10.1016/S0014-5793(01)02613-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The fact that information flows from DNA to RNA to protein to function suggests that regulation is ‘hierarchical’, i.e. dominated by regulation of gene expression. In the case of dominant regulation at the metabolic level, however, there is no quantitative relationship between mRNA levels and function. We here develop a method to quantitate the relative contributions of metabolic and hierarchical regulation. Applying this method to the glycolytic flux in three species of parasitic protists, we conclude that it is rarely regulated by gene expression alone. This casts strong doubts on whether transcriptome and proteome analysis suffices to assess biological function.

【 授权许可】

Unknown