Complex formation and submembranous localization of annexin 2 and S100A10 in live HepG2 cells

FEBS Letters

Gerke, Volker¹  Zobiack, Nicole¹  Rescher, Ursula¹ 

[1] Institute for Medical Biochemistry, ZMBE, University of Münster, von-Esmarch-Str. 56, D-48149 Münster, Germany

关键词: Calcium;    Live cell imaging;    Membrane cytoskeleton;    Membrane transport;    CFP;    cyan fluorescent protein;    YFP;    yellow fluorescent protein;   

DOI  :  10.1016/S0014-5793(01)02604-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Ca²⁺ and membrane binding protein annexin 2 can form a heterotetrameric complex with the S100A10 protein and this complex is thought to serve a bridging or scaffolding function in the membrane underlying cytoskeleton. To elucidate which of the subunits targets the complex to the subplasmalemmal region in live cells we employed YFP/CFP fusion proteins and live cell imaging in HepG2 cells. We show that monomeric annexin 2 is targeted to the plasma membrane whereas non-complexed S100A10 acquires a general cytosolic distribution. Co-expression of S100A10 together with annexin 2 and the resulting complex formation, however, lead to a recruitment of S100A10 to the plasma membrane thus identifying annexin 2 as the membrane targeting subunit.

【 授权许可】

Unknown