A deeper investigation on carbohydrate cycling in Sinorhizobium meliloti

FEBS Letters

Portais, Jean-Charles¹  Gosselin, Isabelle¹  Wattraint, Olivier¹  Barbotin, Jean-Noël¹  Riboul, David¹ 

[1] Laboratoire de Génie Cellulaire, UMR CNRS 6022, Faculté des Sciences, Université de Picardie Jules Verne, 33 rue Saint Leu, 80039 Amiens Cedex, France

关键词: Carbohydrate cycling;    β-Glucan;    Gluconate bypass;    Nuclear magnetic resonance spectroscopy;    Sinorhizobium meliloti;    ED;    Entner–Doudoroff pathway;    EMP;    Embden–Meyerhof–Parnas pathway;    F6P;    fructose-6-phosphate;    G6P;    glucose-6-phosphate;    GAP;    glyceraldehyde-3-phosphate;    GDH;    glucose dehydrogenase;    NMR;    nuclear magnetic resonance;    PFK;    phosphofructokinase;    PGI;    phosphoglucoisomerase;    PP;    pentose-phosphate pathway;   

DOI  :  10.1016/S0014-5793(01)02518-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Recycling of triose-phosphate and pentose-phosphate was previously reported on glucose in Sinorhizobium meliloti, a polysaccharide-synthesizing bacterium, but the metabolic basis of such processes remained unclear. In this work, we have used ¹³C-labelling strategies to demonstrate that carbohydrate cycling in this organism is independent of the gluconate bypass, the alternative pathway for glucose assimilation involving its periplasmic oxidation into gluconate. Furthermore, carbohydrate cycling in S. meliloti is also observed on fructose, making the situation in this bacterium significantly different from that depicted for alginate-synthesizing species.

【 授权许可】

Unknown