Cleavage of mitochondria‐like transfer RNAs expressed in Escherichia coli

FEBS Letters

Delihas, Nicholas¹  Paquin, Bruno²  Bourdeau, Véronique²  Cedergren, Robert² 

[1] Department of Molecular Genetics and Microbiology, School of Medicine, SUNY, Stony Brook, NY 11794-5222, USA;Département de Biochimie, Université de Montréal, C.P. 6128, Succursale Centre-Ville, Montréal, QC, Canada H3C 3J7

关键词: Atypical tRNA;    Mitochondrial tRNA;    tRNA processing;    RNase P;    Escherichia coli;    mt;    mitochondrial;    tRNAs;    transfer RNAs;    PMSF;    phenylmethylsulfonylfluoride;    DTT;    dithiothreitol;   

DOI  :  10.1016/S0014-5793(01)02443-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Mitochondrial (mt) transfer RNAs (tRNAs) often harbor unusual structural features causing their secondary structure to differ from the conventional cloverleaf. tRNAs designed with such irregularities, termed mt-like tRNAs, are active in Escherichia coli as suppressors of reporter genes, although they display low steady-state levels. Characterization of fragments produced during mt-like tRNA processing in vitro and in vivo suggests that these RNAs are not fully processed at their 5′ ends and are cleaved internally. These abnormal processing events may account for the low levels of mature mt-like RNAs in vivo and are most likely related to defective processing by RNase P.

【 授权许可】

Unknown