Characterization of a novel human putative mitochondrial transporter homologous to the yeast mitochondrial RNA splicing proteins 3 and 4

FEBS Letters

Leibiger, Barbara²  Schweyen, Rudolf¹  Nikali, Kaisu³  Leibiger, Ingo²  Gregan, Juraj¹  Li, Fang-Yuan⁴  Larsson, Catharina⁴  Suomalainen, Anu³ 

[1] Vienna Biocenter, Institute of Microbiology and Genetics, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria;Department of Molecular Medicine, The Rolf Luft Center for Diabetes Research L3, Karolinska Hospital, 171 76 Stockholm, Sweden;Department of Human Molecular Genetics, National Public Health Institute, Mannerheimintie 166, FIN-00300 Helsinki, Finland;Department of Molecular Medicine, CMM, L8:01, Karolinska Hospital, 171 76 Stockholm, Sweden

关键词: Mitochondrial carrier;    Solute transport;    MRS3;    MRS4;    Chromosome 10;   

DOI  :  10.1016/S0014-5793(01)02319-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report here a novel human gene, hMRS3/4, encoding a putative mitochondrial transporter structurally and functionally homologous to the yeast mitochondrial RNA splicing proteins 3 and 4. These proteins belong to the family of mitochondrial carrier proteins (MCF) and are likely to function as solute carriers. hMRS3/4 spans ∼10 kb of genomic DNA on chromosome 10q24 and consists of four exons that encode a 364-aa protein with six transmembrane domains. A putative splice variant, encoding a 177-aa protein with three transmembrane domains, was also identified. hMRS3/4 has a well-conserved signature sequence of MCF and is targeted into the mitochondria. When expressed in yeast, hMRS3/4 efficiently restores the mitochondrial functions in mrs3^omrs4^o knock-out mutants. Ubiquitous expression in human tissues and a well-conserved structure and function suggest an important role for hMRS3/4 in human cells.

【 授权许可】

Unknown