Down‐regulation of peroxisome proliferator‐activated receptor‐γ gene expression by sphingomyelins

FEBS Letters

Martinet, Nadine¹  Al-Makdissy, Nehmann³  Younsi, Mohamed³  Dauça, Michel³  Bianchi, Arnaud³  Picard, Evelyne¹  Valet, Philippe²  Donner, Mireille³ 

[1] C.L.E.R.C. Poumon, Faculté de Médecine, Vandoeuvre les Nancy, France;INSERM U 317, Toulouse, France;Laboratoire de Nutrition et Maladies Métaboliques, EA 2402 ‘Proliferateurs de Peroxisomes’, Faculté de Médecine, Université Henri-Poincaré, P.O. Box 184, 54505 Vandoeuvre les Nancy, France

关键词: Sphingomyelin;    Hydrocarbon chain;    Cholesterol;    PPARγ;    Gene expression;   

DOI  :  10.1016/S0014-5793(01)02278-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We recently demonstrated that the sphingomyelin (SM) content of adipocyte membranes was negatively correlated with the expression of peroxisome proliferator-activated receptor-γ (PPARγ) in the subcutaneous adipose tissue of obese women with variable degrees of insulin resistance. We have now investigated whether SM really does have an impact on the expression of PPARγ in 3T3-F442A adipocytes. Adding SM to the culture medium for 24 h caused a significant increase in SM content of adipocyte membranes and an acyl chain length-dependent decrease in the levels of PPARγ mRNA and protein. The longer the acyl chain of the fatty acid of SM, the greater was the decrease in PPARγ. These data suggest that the nature of the fatty acid is important in the regulation of PPARγ by the SM pathway.

【 授权许可】

Unknown