FEBS Letters | |
Sphingolipids of the mycopathogen Sporothrix schenckii: identification of a glycosylinositol phosphorylceramide with novel core GlcNH2α1→2Ins motif | |
Toledo, Marcos S.2  Levery, Steven B.1  Takahashi, Helio K.2  Straus, Anita H.2  | |
[1] Department of Biochemistry and Molecular Biology, and The Complex Carbohydrate Research Center, University of Georgia, 220 Riverbend Road, Athens, GA 30602, USA;Department of Biochemistry, Universidade Federal de São Paulo/Escola Paulista de Medicina, Rua Botucatu 862, 04023-900 São Paulo, SP, Brazil | |
关键词: Sphingolipid; Glycolipid; Dimorphism; Mycopathogen; Nuclear magnetic resonance; Mass spectrometry; NMR; nuclear magnetic resonance; COSY; correlation spectroscopy; TOCSY; total correlation spectroscopy; NOESY; nuclear Overhauser effect spectroscopy; HPLC; high performance liquid chromatography; HPTLC; high performance thin layer chromatography; GC; gas chromatography; ESI; electrospray ionization; MS; mass spectrometry; CID; collision-induced decomposition; DMSO; dimethyl sulfoxide; TMS; trimethylsilyl; FAME; fatty acid methyl ester; | |
DOI : 10.1016/S0014-5793(01)02275-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Acidic glycosphingolipid components were extracted from the yeast form of the dimorphic mycopathogen Sporothrix schenckii. Two minor and the major fraction from the yeast form (Ss-Y1, -Y2, and -Y6, respectively) have been isolated. By a combination of 1- and 2-D 1H-nuclear magnetic resonance (NMR) spectroscopy, electrospray ionization mass spectrometry (ESI-MS), and gas chromatography/mass spectrometry (GC/MS), Ss-Y6 was determined to be triglycosylinositol phosphorylceramide with a novel glycan structure, Manα1→3Manα1→6GlcNH2α1→2Ins1-P-1Cer (where Ins=myo-inositol, P=phosphodiester). While the GlcNH2α1→ 6 Ins1-P- motif is found widely distributed in eukaryotic GPI anchors, the linkage GlcNH2α1→ 2 Ins1-P- has not been previously observed in any glycolipid. Ss-Y1 and Ss-Y2 were both found to have the known glycan structure Manα1→3Manα1→2Ins1-P-1Cer. Together with the results of a prior study [Toledo et al. (2001) Biochem. Biophys. Res. Commun. 280, 19–24] which showed that the mycelium form expresses GIPCs with the structures Manα1→6Ins1-P-1Cer and Manα1→3Manα1→6Ins1-P-1Cer, these results demonstrate that S. schenckii can synthesize glycosylinositol phosphorylceramides with at least three different core linkages.
【 授权许可】
Unknown
【 预 览 】
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