A cis‐dominant cyclic nucleotide‐dependent regulatory domain in the 3′‐untranslated region of Na+/glucose cotransporter (SGLT1) mRNA

FEBS Letters

Loflin, Paul¹  Lever, Julia E¹ 

[1] Department of Biochemistry and Molecular Biology, University of Texas-Houston Medical School, P.O. Box 20708, Houston, TX 77225, USA

关键词: mRNA stability;    cAMP;    Glucose transporter;    SGLT;    Na+-coupled glucose transporter;    GAPDH;    glyceraldehyde-3-phosphate dehydrogenase;    IBMX;    3-isobutyl-1-methylxanthine;    UTR;    untranslated region;    URE;    U-rich element;   

DOI  :  10.1016/S0014-5793(01)02260-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A 122 nt uridine-rich sequence (URE) in the Na⁺/glucose cotransporter (SGLT1) mRNA 3′-untranslated region is critical for cAMP-dependent message stabilization. Its function was investigated in LLC-PK₁ cells stably expressing β-globin reporter transcripts. Insertion of the SGLT1 URE downstream from an unrelated destabilizing sequence, the c-fos ARE, evoked cAMP-dependent message stabilization. Stabilization was blocked by a substitution mutation within the SLGT1 URE. These observations indicate that the SGLT1 URE is sufficient to transmit cAMP-dependent, cis-dominant mRNA stabilization in the presence of appropriate trans-acting factors and appears to function independently of the nature of the destabilizing domain.

【 授权许可】

Unknown