Inhibition of gap junctional intercellular communication in rat liver epithelial cells with transforming RNA

FEBS Letters

Trosko, James E.²  Hamada, Katsutomo¹  Hayashi, Tomonori² 

[1] Division of Cell Biology, Research Institute for Radiation Biology and Medicine, Hiroshima University, 1-2-3 Kasumi, Minami Ward, Hiroshima 734-0037, Japan;Department of Pediatrics/Human Development, Institute of Environmental Toxicology and the Cancer Center of MSU, Michigan State University, 246 NFST Bldg., East Lansing, MI 48824, USA

关键词: Gap junctional intercellular communication;    Connexin 43;    Transforming RNA;    Rat liver epithelial cell;    Cx43;    connexin 43;    FRAP;    fluorescence redistribution after photobleaching;    GJIC;    gap junctional intercellular communication;    PBS;    phosphate-buffered saline;    PCR;    polymerase chain reaction;    RT;    reverse transcription;    SDS;    sodium dodecyl sulfate;    SRP;    signal recognition particle;   

DOI  :  10.1016/S0014-5793(01)02185-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previous studies indicated that transforming RNA, derived from the 3′ half of the U5 small nuclear RNA first stem structure, suppressed the secretory protein translation in vitro. Gap junctions facilitate homeostatic control of cell growth and differentiation and their dysfunction has been correlated with carcinogenesis. Here, we reported that transforming RNA directly suppressed the gap junction protein, connexin 43, translation and thereby inhibited functional gap junction function in rat epithelial cells. Together with previous data, this implies that altered expression of transforming RNA itself is a potential mechanism in inhibiting gap junction function during carcinogenesis.

【 授权许可】

Unknown