A novel patatin‐like gene stimulated by drought stress encodes a galactolipid acyl hydrolase

FEBS Letters

Kader, Jean-Claude²  Edelman, Léna³  Zuily-Fodil, Yasmine¹  d'Arcy-Lameta, Agnès¹  Matos, Ana Rita¹  Pêtres, Stéphane³  Pham-Thi, Anh Thu¹  França, Marcel¹ 

[1] Laboratoire de Biochimie et Physiologie de l'Adaptation Végétale, Université Denis Diderot, case 7019, 2 Place Jussieu, 75251 Paris Cedex 05, France;Laboratoire de Physiologie Cellulaire et Moléculaire des Plantes, Université Pierre et Marie Curie, 4 Place Jussieu, 75252 Paris, France;Laboratoire de Technologie Cellulaire, Département des Biotechnologies, Institut Pasteur, 25 rue du Dr Roux, 75015 Paris, France

关键词: Drought stress;    Lipolytic acyl hydrolase;    Galactolipase;    Patatin;    Cowpea (Vigna unguiculata L. Walp);    LAH;    lipolytic acyl hydrolase;    MGDG;    monogalactosyldiacylglycerol;    DGDG;    digalactosyldiacylglycerol;    SQDG;    sulphoquinovosyldiacylglycerol;    PC;    phosphatidylcholine;    PLA2;    phospholipase A2;    FA;    fatty acids;   

DOI  :  10.1016/S0014-5793(01)02194-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA (Vupat1) encoding a predicted 43 kDa protein was isolated from drought-stressed cowpea (Vigna unguiculata) leaves. It has homology with patatin, a potato tuber storage protein with lipolytic acyl hydrolase activity. The recombinant protein VUPAT1 expressed in the baculovirus system displays preferentially galactolipid acyl hydrolase activity. Phospholipids are very slowly hydrolyzed and apparently triacylglycerols are not deacylated. Vupat1 promoter contains putative drought-inducible sequences. Northern blots showed that gene expression is stimulated by drought stress and is more pronounced in a drought-sensitive cultivar than in a drought-tolerant one. An involvement in drought-induced galactolipid degradation is proposed for VUPAT1.

【 授权许可】

Unknown