c‐IAP2 is induced by ionizing radiation through NF‐κB binding sites

FEBS Letters

Hiraoka, Masahiro¹  Noda, Makoto²  Ueda, Toshihiko²  Kizaka-Kondoh, Shinae²  Sai, Heitetsu¹  Akiyama, Nobutate²  Oya, Natsuo¹ 

[1] Department of Therapeutic Radiology and Oncology, Kyoto University Graduate School of Medicine, 54 Kawahara-cho, Shogoin, Sakyo-ku, Kyoto 606-8507, Japan;Department of Molecular Oncology, Kyoto University Graduate School of Medicine, Yoshida-Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan

关键词: Gene trap;    c-IAP2/HIAP-1/hMIHC;    Radiation;    Nuclear factor-κB;    BAX;    Apoptosis;    NF-κB;    nuclear factor-κB;    NFB;    NF-κB binding sites;    IAP;    inhibitors of apoptosis;    BLM;    bleomycin hydrochloride;   

DOI  :  10.1016/S0014-5793(01)02145-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transcriptional promoters responsive to low doses of X-irradiation may be useful in developing a new strategy in gene therapy combined with conventional radiotherapy. The retrovirus-mediated gene trap screening identified c-IAP2 as one of genes possessing such promoters. The analysis of the cis-elements responsive to X-irradiation in c-IAP2 promoter revealed that the NF-κB binding sites were necessary and sufficient for the X-ray-responsiveness. We constructed the plasmid p4NFB-BAX, which had four tandem repeats of the NF-κB binding sites of c-IAP2 promoter (4NFB) and a suicide gene BAX under the control of 4NFB. The human tumor cells transfected with p4NFB-BAX significantly reduced the number of cells that survived 2 Gy irradiation.

【 授权许可】

Unknown