An Arabidopsis T‐DNA mutant affected in Nrt2 genes is impaired in nitrate uptake

FEBS Letters

Filleur, Sophie³  Cerezo, Miguel¹  Dorbe, Marie-France³  Gojon, Alain¹  Granier, Fabienne²  Daniel-Vedele, Françoise³  Orsel, Mathilde³ 

[1] Biochimie et Physiologie Moléculaire des Plantes, UMR 50004, AGRO-M/CNRS/INRA/UM2, Place Viala, F-34060 Montpellier Cedex, France;Laboratoire de Biologie Cellulaire, INRA, Route de St. Cyr, F-78026 Versailles Cedex, France;Unité de la Nutrition Azotée des Plantes, INRA, Route de St. Cyr, F-78026 Versailles Cedex, France

关键词: Nitrate transport;    T-DNA mutant;    Complementation;    Arabidopsis;   

DOI  :  10.1016/S0014-5793(01)02096-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression analyses of Nrt2 plant genes have shown a strict correlation with root nitrate influx mediated by the high-affinity transport system (HATS). The precise assignment of NRT2 protein function has not yet been possible due to the absence of heterologous expression studies as well as loss of function mutants in higher plants. Using a reverse genetic approach, we isolated an Arabidopsis thaliana knock-out mutant where the T-DNA insertion led to the complete deletion of the AtNrt2.1 gene together with the deletion of the 3′ region of the AtNrt2.2 gene. This mutant is impaired in the HATS, without being modified in the low-affinity system. Moreover, the de-regulated expression of a Nicotiana plumbaginifolia Nrt2 gene restored the mutant nitrate influx to that of the wild-type. These results demonstrate that plant NRT2 proteins do have a role in HATS.

【 授权许可】

Unknown