Transformation of Saccharomyces cerevisiae with a cDNA encoding a sterol C‐methyltransferase from Arabidopsis thaliana results in the synthesis of 24‐ethyl sterols

FEBS Letters

Bard, Martin²  Desprez, Thierry³  Benveniste, Pierre¹  Gachotte, Daniel¹  Husselstein, Tania¹ 

[1] Institut de Biologie Moléculaire des Plantes, Département de Biologie Cellulaire et Moléculaire, Institut de Botanique, 28 rue Goethe, 67083 Strasbourg Cédex, France;IUPUI, Indiana University-Purdue University at Indianapolis, 723 W. Michigan Street, Indianapolis, IN 46202, USA;Laboratoire de Biologie Cellulaire, Institut National de la Recherche Agronomique, route de St-Cyr, 78026 Versailles, France

关键词: Arabidopsis;    S-Adenosylmethionine-sterol-C-methyltransferase;    erg6;    Complementation;    I = campesterol = (24ζ)-24-methyl-cholest-5-en-3β-ol;    II = sitosterol = (24R)-24-ethyl-cholest-5-en-3β-ol;    III = zymosterol = 5α-cholesta-8;    24-dien-3β-ol;    IV = fecosterol = 5α-ergosta-8;    24(241)-dien-3β-ol;    V = cycloartenol =14α-trimethyl-9β;    9-cyclo-5α-cholest-24-en-3β-ol;    VI = 24-methylene cycloartanol = 4;    4;    14α-trimethyl-9β;    19-cyclo-5α-ergost-24(241)-en-3β-ol;    VII = 24-methylene lophenol = 4α-methyl-5α-ergosta-7;    24(241)-dien-3β-ol;    VII = 24-ethyliden lophenol = 4α-methyl-5α-stigmasta-7-Z-24(241)-dien-3β-ol;    IX = ergosterol = ergosta-5;    7;    E-22-trien-3β-ol;    X = Δ 7-avenasterol = 5α-stigmasta-7;    Z-24(241)-dien-3β-ol;    XII = stigmasta-5;    7;    E-22-trien-3β-ol;    XIV = 5α-cholesta-7;    24-dien-3β-ol;    XV = cholesta-5;    7;    8;    -tetraten-3β-ol;    XVI = lanosterol = 4;    4;    14α-trimethyl-5α-cholesta-8;    24-dien-4β-ol;    XVIII = ergosta;    5;    7;    9;    E-22-tetraen-3β-ol;    XVIII = eburicol =α-trimethyl-5α-egosta-8;    24(241)-dien-3β-ol;   

DOI  :  10.1016/0014-5793(96)00089-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using an EST-cDNA probe, a full-length cDNA (411) sequence of 1411 bp was isolated from A. thaliana. This sequence contained features typical of methyltransferases in general and in particular showed 38% identity with ERG6, a S. cerevisiae gene which encodes the zymosterol-C-24-methyltransferase. A yeast vector containing this ORF (4118-pYeDP60) was used to transform a wild type S. cerevisiae which accumulates predominantly ergosterol, a 24-methyl sterol as well as a mutant erg6 null mutant accumulating principally zymosterol, a sterol non-alkylated at C-24. In both cases, several 24-ethyl- and 24-ethylidene sterols were synthetized indicating that the 4118 cDNA encodes a plant sterol C-methyltransferase able to perform two sequential methylations of the sterol side chain.

【 授权许可】

Unknown