Prolactin activation of IRF‐1 transcription involves changes in histone acetylation

FEBS Letters

Yu-Lee, Li-yuan¹  Book McAlexander, Melissa¹ 

[1] Department of Molecular and Cellular Biology, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX 77030 USA

关键词: Chromatin immunoprecipitation;    H4 acetylation;    Interferon regulatory factor-1;    Transcription;    Stat1;    Prolactin;   

DOI  :  10.1016/S0014-5793(00)02385-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In response to prolactin (PRL) signaling, transcription of the interferon regulatory factor-1 gene (IRF-1) is rapidly induced during early G₁, declines in mid G₁, and rises again over the G₁/S transition phase of the cell cycle in Nb2 T cells. Using chromatin immunoprecipitation assays, we show that histone H4 acetylation increases over a 1.7 kb region of the IRF-1 promoter in early G₁ and again at the G₁/S transition in response to PRL stimulation. These results demonstrate a correlation between histone H4 hyperacetylation at the IRF-1 promoter and biphasic transcription of IRF-1 in response to PRL signaling in vivo.

【 授权许可】

Unknown