FEBS Letters | |
SRC transcriptional activation in a subset of human colon cancer cell lines | |
Senger, Mary-Anne2  Dehm, Scott1  Bonham, Keith2  | |
[1] Department of Biochemistry, University of Saskatchewan, Saskatoon, Sask., Canada S7N 5E5;Cancer Research Unit, Health Research Division, Saskatchewan Cancer Agency, and the Division of Oncology, University of Saskatchewan, Saskatoon, Sask., Canada S7N 4H4 | |
关键词: c-Src; Oncogene activation; Transcription; Colon cancer; HCCL; human colon cancer cell line; CRC; colorectal cancer; EGFR; epidermal growth factor receptor; | |
DOI : 10.1016/S0014-5793(00)02354-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Human SRC encodes the non-receptor tyrosine kinase pp60c-Src, which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.
【 授权许可】
Unknown
【 预 览 】
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RO201912020310148ZK.pdf | 207KB | download |