SRC transcriptional activation in a subset of human colon cancer cell lines

FEBS Letters

Senger, Mary-Anne²  Dehm, Scott¹  Bonham, Keith² 

[1] Department of Biochemistry, University of Saskatchewan, Saskatoon, Sask., Canada S7N 5E5;Cancer Research Unit, Health Research Division, Saskatchewan Cancer Agency, and the Division of Oncology, University of Saskatchewan, Saskatoon, Sask., Canada S7N 4H4

关键词: c-Src;    Oncogene activation;    Transcription;    Colon cancer;    HCCL;    human colon cancer cell line;    CRC;    colorectal cancer;    EGFR;    epidermal growth factor receptor;   

DOI  :  10.1016/S0014-5793(00)02354-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human SRC encodes the non-receptor tyrosine kinase pp60^c-Src, which is activated in many human colon cancer cell lines (HCCLs) and tumors. We found that both c-Src protein and mRNA levels were elevated in a subset of HCCLs. Increased c-Src mRNA and protein levels correlated strongly with increased c-Src kinase activity. Nuclear run-on analysis and c-Src mRNA half-life determination demonstrated increased mRNA levels were due to increased transcription of the SRC gene. We also observed decreased c-Src mRNA stability in cell lines that displayed SRC transcriptional activation. Our findings provide the first evidence that SRC transcriptional activation is an important determinant of c-Src expression and activity in HCCLs.

【 授权许可】

Unknown