FEBS Letters | |
Cellular retinoic acid binding protein is associated with mitochondria | |
Ruff, Susan J1  Ong, David E1  | |
[1] Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA | |
关键词: Cellular retinoic acid-binding protein; Vitamin A; Retinoid; Mitochondrion; Targeting; CRABP; cellular retinoic acid binding protein; CRBP; cellular retinol-binding protein; RA; retinoic acid; | |
DOI : 10.1016/S0014-5793(00)02366-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We report that immunohistochemical staining for cellular retinoic acid-binding protein (CRABP) was restricted to the cytoplasm of cortical cells in bovine adrenal. In contrast, staining for the similar protein, cellular retinol-binding protein (CRBP), was found throughout these cells. After transfections of CRABP and CRBP into cultured cells, immunofluorescence analyses again revealed cytoplasmic restriction only for CRABP, with a pronounced punctate appearance. Use of organelle-specific fluorochromes indicated that CRABP immunofluorescence overlaid exactly with the pattern of the mitochondrial-specific fluorochrome. Confirmation of this association came with subcellular fractionation of the adrenal cortex. CRABP, but not CRBP, co-sedimented with the mitochondria, a novel finding for a member of this superfamily of cellular lipid-binding proteins.
【 授权许可】
Unknown
【 预 览 】
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RO201912020310132ZK.pdf | 438KB | download |