Cellular retinoic acid binding protein is associated with mitochondria

FEBS Letters

Ruff, Susan J¹  Ong, David E¹ 

[1] Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37232, USA

关键词: Cellular retinoic acid-binding protein;    Vitamin A;    Retinoid;    Mitochondrion;    Targeting;    CRABP;    cellular retinoic acid binding protein;    CRBP;    cellular retinol-binding protein;    RA;    retinoic acid;   

DOI  :  10.1016/S0014-5793(00)02366-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report that immunohistochemical staining for cellular retinoic acid-binding protein (CRABP) was restricted to the cytoplasm of cortical cells in bovine adrenal. In contrast, staining for the similar protein, cellular retinol-binding protein (CRBP), was found throughout these cells. After transfections of CRABP and CRBP into cultured cells, immunofluorescence analyses again revealed cytoplasmic restriction only for CRABP, with a pronounced punctate appearance. Use of organelle-specific fluorochromes indicated that CRABP immunofluorescence overlaid exactly with the pattern of the mitochondrial-specific fluorochrome. Confirmation of this association came with subcellular fractionation of the adrenal cortex. CRABP, but not CRBP, co-sedimented with the mitochondria, a novel finding for a member of this superfamily of cellular lipid-binding proteins.

【 授权许可】

Unknown