Matrix Gla protein is differentially expressed during the deposition of a calcified matrix by vascular pericytes

FEBS Letters

Canfield, Ann E.²  Kelly, Victoria¹  Boot-Handford, Raymond P.¹  Grant, Michael E.¹  Doherty, Mary Jo²  Farrington, Claire²  Newman, Bill² 

[1] Wellcome Trust Centre for Cell-Matrix Research, School of Biological Sciences, University of Manchester, 2.205, Stopford Building, Oxford Road, Manchester M13 9PT, UK;Wellcome Trust Centre for Cell-Matrix Research, School of Medicine, University of Manchester, 2.205, Stopford Building, Oxford Road, Manchester M13 9PT, UK

关键词: Matrix Gla protein;    Pericyte;    Calcification;   

DOI  :  10.1016/S0014-5793(00)02363-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

PCR-based subtractive hybridisation was used to identify genes up-regulated when pericytes undergo osteogenic differentiation and deposit a calcified matrix. cDNA pools were generated from confluent pericytes and from pericyte cultures containing calcified nodules. A pericyte cDNA library was screened with the product of the subtraction procedure (calcified minus confluent cDNA) and the majority of the positive clones were identified as matrix Gla protein (MGP). Northern analysis and immunohistochemistry demonstrated that MGP was only expressed by pericytes in calcified nodules. Antibodies to MGP inhibited the deposition of a calcified matrix by pericytes, suggesting that MGP regulates both cell differentiation and calcification.

【 授权许可】

Unknown