Genomic Exploration of the Hemiascomycetous Yeasts: 19. Ascomycetes‐specific genes

FEBS Letters

Bolotin-Fukuhara, Monique²  Souciet, Jean-Luc³  Casarégola, Serge¹  Dujon, Bernard⁴  Saurin, William⁶  Aigle, Michel⁵  Bon, Elisabeth¹  Potier, Serge³  Weissenbach, Jean⁶  Lépingle, Andrée¹  Wésolowski-Louvel, Micheline⁷  Neuvéglise, Cécile¹  Artiguenave, Francois⁶  Wincker, Patrick⁶  Gaillardin, Claude¹  Ozier-Kalogeropoulos, Odile⁴  Malpertuy, Alain⁴  Tekaia, Fredj⁴  Durrens, Pascal⁵  Brottier, Philippe⁶  Blandin, Gaëlle⁴  de Montigny, Jacky³  Llorente, Bertrand⁴  Toffano-Nioche, Claire² 

[1] Collection de Levures d'Intéret Biotechnologique, Laboratoire de Génétique Moléculaire et Cellulaire, INRA UMR216, CNRS URA1925, INA-PG, BP01, F-78850 Thiverval-Grignon, France;Institut de Génétique Moléculaire, CNRS/UPS UMR 8621, Batiment 400, Université de Paris Sud, F-91405 Orsay, France;Laboratoire de Génétique et Microbiologie, UPRES-A 7010 ULP/CNRS, Institut de Botanique, 28 rue Goethe, F-67000 Strasbourg Cedex, France;Unité de Génétique Moléculaire des Levures, URA 2171 CNRS and UFR927 Université P. et M. Curie, Institut Pasteur, 25 Rue du Dr Roux, F-75724 Paris Cedex 15, France;Laboratoire de Biologie cellulaire de la Levure, IBGC, 1 rue Camille Saint-Saens, F-33077 Bordeaux Cedex, France;Génoscope, Centre National de Séquenage, 2 rue Gaston Crémieux, BP191, Evry Cedex, France;Microbiologie et Génétique, CNRS/UCB/INSA ERS 2009, Bat. 405 R2, Université Lyon I, F-69622 Villeurbanne Cedex, France

关键词: Orphan;    Maverick;    Questionable open reading frame;    Novel gene;    Gene number;   

DOI  :  10.1016/S0014-5793(00)02290-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Comparisons of the 6213 predicted Saccharomyces cerevisiae open reading frame (ORF) products with sequences from organisms of other biological phyla differentiate genes commonly conserved in evolution from ‘maverick’ genes which have no homologue in phyla other than the Ascomycetes. We show that a majority of the ‘maverick’ genes have homologues among other yeast species and thus define a set of 1892 genes that, from sequence comparisons, appear ‘Ascomycetes-specific’. We estimate, retrospectively, that the S. cerevisiae genome contains 5651 actual protein-coding genes, 50 of which were identified for the first time in this work, and that the present public databases contain 612 predicted ORFs that are not real genes. Interestingly, the sequences of the ‘Ascomycetes-specific’ genes tend to diverge more rapidly in evolution than that of other genes. Half of the ‘Ascomycetes-specific’ genes are functionally characterized in S. cerevisiae, and a few functional categories are over-represented in them.

【 授权许可】

Unknown