A new family of orphan G protein‐coupled receptors predominantly expressed in the brain

FEBS Letters

Breu, Volker¹  Giller, Thomas¹  Richards, J.Grayson¹  Ardati, Ali¹  Valdenaire, Olivier¹  Schweizer, Anja¹ 

[1] Pharma Division, Preclinical Research, F. Hoffmann-La Roche Ltd., CH-4070 Basel, Switzerland

关键词: Endothelin;    Orphan;    G protein-coupled receptor;    ETBR-LP;    ETB receptor-like protein;    EST;    expressed sequence tag;   

DOI  :  10.1016/S0014-5793(98)00170-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The cloning of a cDNA encoding a G protein-coupled receptor homologous to the endothelin type B receptor, but unable to bind endothelin, was recently reported and termed ET_BR-LP. We report here the isolation of a human cDNA encoding a receptor that is highly related to ET_BR-LP and which was therefore termed ET_BR-LP-2. Comparison of the two amino acid sequences revealed 68% overall homology and 48% identity. As is the case for ET_BR-LP, the new receptor is strongly expressed in the human central nervous system (e.g. in cerebellar Bergmann glia, cerebral cortex, internal capsule fibers). Membranes of HEK-293 cells stably expressing ET_BR-LP-2 did not bind endothelin-1, endothelin-2, endothelin-3, bombesin, cholecystokinin-8 or gastrin-releasing peptide.

【 授权许可】

Unknown