Peroxisome proliferator‐activated receptor γ activators inhibit interleukin‐12 production in murine dendritic cells

FEBS Letters

Fontaine, Josette⁴  Staels, Bart²  Maliszewski, Charlie⁵  Chinetti, Giulia²  Angeli, Véronique⁴  Faveeuw, Christelle⁴  Delerive, Philippe²  Trottein, François⁴  Gosset, Philippe¹  Moser, Muriel³  Fougeray, Sylvie³  Capron, Monique⁴ 

[1]INSERM U416, Institut Pasteur de Lille, 59019 Lille, France

[2]INSERM U325, Institut Pasteur de Lille, 59019 Lille, France

[3]Université Libre de Bruxelles, Gosselies, Belgium

[4]INSERM U167, Institut Pasteur de Lille, 59019 Lille, France

[5]Immunex Corp., Seattle, WA, USA

关键词: Dendritic cell;    Peroxisome proliferator-activated receptor;    Interleukin-12;    Abs;    antibodies;    APCs;    antigen presenting cells;    DCs;    dendritic cells;    15d-PGJ2;    15-deoxy-Δ12;    14-prostaglandin J2;    KLH;    keyhole limpet hemocyanin;    LNs;    lymph nodes;    PPARs;    peroxisome proliferator-activated receptors;    RSG;    rosiglitazone;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    Th;    T helper;   

DOI  :  10.1016/S0014-5793(00)02319-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Peroxisome proliferator-activated receptors (PPARs) are members of the nuclear receptor superfamily. They are divided into three subtypes (α, β or δ, and γ) and are involved in lipid and glucose homeostasis and in the control of inflammation. In this study, we analyzed the expression of PPARs in murine dendritic cells (DCs), the most potent antigen presenting cells. We find that immature as well as mature spleen-derived DCs express PPARγ, but not PPARα, mRNA and protein. We also show that the PPARγ activator rosiglitazone does not interfere with the maturation of DCs in vitro nor modifies their ability to activate naive T lymphocytes in vivo. Finally, we present evidence that PPARγ activators down-modulate the CD40-induced secretion of interleukin-12, a potent Th1-driving factor. These data suggest a possible role for PPARγ in the regulation of immune responses.

【 授权许可】

Unknown