Interleukin‐12 secreted by mature dendritic cells mediates activation of NK cell function

FEBS Letters

Khar, Ashok¹  Alli, Rajshekhar S¹ 

[1] Centre for Cellular and Molecular Biology, Uppal Road, Hyderabd 500 007, India

关键词: Dendritic cell;    Natural killer cell;    Cytotoxicity;    Activation;    Interleukin-12;   

DOI  :  10.1016/S0014-5793(04)00026-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Dendritic cells (DCs) are known to modulate immune response by activating effector cells of both the innate and the adaptive immune system. In the present study, we demonstrate that co-culture of DCs with paraformaldehyde-fixed tumor cells augments the secretion of interleukin (IL)-12 by DCs and these activated DCs upon co-culture with naive NK cells enhance the cytolytic activity of NK cells against NK-sensitive target YAC-1. Similarly, DCs isolated from tumor-bearing animals also activated NK cells in vitro. For efficient activation of NK cells, the ratio of activated DCs to NK cells is crucial. Addition of anti-IL-12 antibody to the culture system completely abolished activation of NK cells by DCs, suggesting that IL-12 secreted by DCs is an essential factor in NK cell activation. Adoptive transfer of DCs isolated from tumor-bearing animals into normal rats also induced activation of NK cells in normal animals.

【 授权许可】

Unknown