Effects of homocysteine on the binding of extracellular‐superoxide dismutase to the endothelial cell surface

FEBS Letters

Yamamoto, Masayuki¹  Adachi, Tetsuo¹  Hara, Hirokazu¹ 

[1] Laboratory of Clinical Pharmaceutics, Gifu Pharmaceutical University, 5-6-1 Mitahora-higashi, Gifu 502-8585, Japan

关键词: Homocysteine;    Extracellular-superoxide dismutase;    Endothelial cell;    Heparan sulfate proteoglycan;    Atherosclerosis;   

DOI  :  10.1016/S0014-5793(00)02260-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Homocysteine is known to be a risk factor for several vascular diseases. Previously, we found a significant association between plasma homocysteine and plasma extracellular-superoxide dismutase (EC-SOD) levels. The binding of EC-SOD to human and bovine aortic endothelial cell cultures showed significant decreases after incubation with 10 μM homocysteine, whereas the expression of EC-SOD in fibroblast cell cultures was inhibited with a high concentration (1 mM) of homocysteine. Furthermore, binding of EC-SOD to heparin immobilized on plates was decreased with homocysteine. These observations suggested that homocysteine decreases the binding of EC-SOD to vascular endothelial cell surfaces by degradation of endothelial heparan sulfate proteoglycan, which results in a loss of the ability to protect endothelial cell surfaces from oxidative stress.

【 授权许可】

Unknown