PA‐FABP, a novel marker of human epidermal transit amplifying cells revealed by 2D protein gel electrophoresis and cDNA array hybridisation

FEBS Letters

Frischauf, Anna-Maria¹  Watt, Fiona M.²  Celis, Julio E.³  O'Shaughnessy, Ryan F.L.²  Seery, John P.² 

[1] Institut für Genetik und Allgemeine Biologie, Universität Salzburg, Hellbrunnerstrasse 34, A-5020 Salzburg, Austria;Keratinocyte Laboratory, Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, London WC2A 3PX, UK;Danish Centre for Human Genome Research, Building 170, Ole Worms Alle, DK-8000 Aarhus C, Denmark

关键词: PA-FABP;    Proteomics;    Epidermis;    Stem cell;    Differentiation;    cDNA array hybridisation;   

DOI  :  10.1016/S0014-5793(00)02252-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Human epidermal stem cells express higher levels of β1 integrins than their more differentiated daughters, transit amplifying cells. In a search for additional stem and transit cell markers we used proteomics and differential cDNA hybridisation to compare keratinocytes fractionated on the basis of β1 integrin expression. There were remarkably few differences between the two populations and none of the RNAs differed in abundance by more than 2-fold. Nevertheless, proteomics revealed upregulated expression of epidermal fatty acid binding protein (PA-FABP, also known as E-FABP), Annexin II and two keratin related proteins in the transit population. An unknown high molecular mass protein was upregulated in the stem cell population. The upregulation of PA-FABP was confirmed by Northern blotting and conventional and whole mount labelling of human epidermis. We conclude that PA-FABP is a novel marker of epidermal transit amplifying cells.

【 授权许可】

Unknown