FEBS Letters | |
Characterization of two distinct DP‐related genes from Arabidopsis thaliana 1 | |
Atanassova, Ana1  Inzé, Dirk1  Rombauts, Stéphane1  De Veylder, Lieven1  Magyar, Zoltán1  | |
[1] Vakgroep Moleculaire Genetica, Departement Plantgenetica, Vlaams Interuniversitair voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium | |
关键词: DP; E2F; G1/S transition; Arabidopsis thaliana; CDK; cyclin-dependent serine/threonine protein kinase; HA; influenza hemagglutinin; NLS; nuclear localized signal; Rb; retinoblastoma; RT-PCR; reverse transcription-polymerase chain reaction; | |
DOI : 10.1016/S0014-5793(00)02238-9 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP-related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP-related genes, AtDPa and AtDPb. They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F-related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.
【 授权许可】
Unknown
【 预 览 】
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