Characterization of two distinct DP‐related genes from Arabidopsis thaliana 1

FEBS Letters

Atanassova, Ana¹  Inzé, Dirk¹  Rombauts, Stéphane¹  De Veylder, Lieven¹  Magyar, Zoltán¹ 

[1] Vakgroep Moleculaire Genetica, Departement Plantgenetica, Vlaams Interuniversitair voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium

关键词: DP;    E2F;    G1/S transition;    Arabidopsis thaliana;    CDK;    cyclin-dependent serine/threonine protein kinase;    HA;    influenza hemagglutinin;    NLS;    nuclear localized signal;    Rb;    retinoblastoma;    RT-PCR;    reverse transcription-polymerase chain reaction;   

DOI  :  10.1016/S0014-5793(00)02238-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

E2F/DP complexes play a pivotal role in the regulation of the G1/S transition in animals. Recently, plant E2F homologs have been cloned, but DP-related sequences have not been identified so far. Here we report that Arabidopsis thaliana contains at least two different DP-related genes, AtDPa and AtDPb. They exhibit an overall domain organization similar to that of their animal counterparts, although phylogenetic analysis demonstrated that they form a separate subgroup. AtDPs efficiently heterodimerize in vitro with the Arabidopsis E2F-related proteins, AtE2Fa and AtE2Fb through their dimerization domains. AtDPa and AtE2Fa are predominantly produced in actively dividing cells with highest transcript levels in early S phase cells.

【 授权许可】

Unknown