The metal‐free hydrogenase from methanogenic archaea: evidence for a bound cofactor

FEBS Letters

Shima, Seigo¹  Buurman, Gerrit¹  Thauer, Rudolf K¹ 

[1] Max-Planck-Institut für terrestrische Mikrobiologie and Laboratorium für Mikrobiologie des Fachbereichs Biologie der Philipps-Universität, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany

关键词: Hydrogenase;    H2-forming methylenetetrahydromethanopterin dehydrogenase;    Methanogenic archaea;    Methanobacterium thermoautotrophicum;    Methanococcus jannaschii;    Methanopyrus kandleri;    Hmd;    H2-forming methylenetetrahydromethanopterin dehydrogenase or metal-free hydrogenase;    H4MPT;    tetrahydromethanopterin;    Fmoc;    fluoren-9-ylmethoxycarbonyl;   

DOI  :  10.1016/S0014-5793(00)02225-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The hmd gene, which encodes the metal-free hydrogenase in methanogenic archaea, was heterologously expressed in Escherichia coli. The overproduced enzyme was completely inactive. High activity could, however, be induced by the addition of ultrafiltrate from active enzyme denatured in 8 M urea. The active fraction in the ultrafiltrate was heat-labile and migrated on gel filtration columns with an apparent molecular mass well below 1000 Da.

【 授权许可】

Unknown