H2‐forming N 5,N 10‐methylenetetrahydromethanopterin dehydrogenase: mechanism of H2 formation analyzed using hydrogen isotopes

FEBS Letters

Fernández, Victor M.¹  Thauer, Rudolf K.²  Klein, Andreas R.² 

[1] Unidad de Biocatalisis, Instituto de Catalisis C.S.I.C., Universidad Autónoma, Cantoblanco, E-28049 Madrid, Spain;Laboratorium für Mikrobiologie, Fachbereich Biologie, Philipps-Universität Marburg, Karl-von-Frisch-Strasse, D-35043 Marburg, Germany

关键词: Hydrogenase;    Tetrahydromethanopterin;    Enzyme mechanism;    Methanogenic Archaea;    Methanobacterium thermoautotrophicum;   

DOI  :  10.1016/0014-5793(95)00642-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

H₂-forming N ⁵,N ¹⁰-methylenetetrahydromethanopterin dehydrogenase catalyzes the reversible dehydrogenation of N ⁵,N ¹⁰-methylenetetrahydromethanopterin (CH₂ = H₄MPT) to N ⁵,N ¹⁰-methenyltetrahydromethanopterin (CH = H₄MPT⁺) and H₂. In D₂O both HD and D₂ are formed from CH₂ = H₄MPT and in H₂O both HD and H₂ from CD₂ = H₄MPT. Evidence is presented that HD is not an intermediate in the formation of D₂ and H₂, respectively.

【 授权许可】

Unknown