Chloroplast development in Arabidopsis thaliana requires the nuclear‐encoded transcription factor Sigma B

FEBS Letters

Yokota, Akiho⁵  Shirano, Yumiko³  Shimada, Hiroshi¹  Takahashi, Hideo⁴  Fujiwara, Makoto⁴  Kanamaru, Kengo⁴  Unno, Kazutoshi²  Shibata, Daisuke³  Hayashi, Hiroaki⁶  Sato, Shuusei⁷  Miyake, Chikahiro⁵  Tanaka, Kan⁴  Tabata, Satoshi⁷ 

[1] Department of Biological Sciences, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Nagatsuta, Midoriku, Yokohama 226-0026, Japan;Department of Orthopedic Surgery, University Hospital, 74 Mizonokuchi, Takatsu-ku Kawasaki, Kanagawa 213-0001, Japan;Mitsui Plant Biotechnology Research Institute, Tsukuba, Ibaraki 305-0047, Japan;Institute of Molecular and Cellular Biosciences, University Hospital, 74 Mizonokuchi, Takatsu-ku Kawasaki, Kanagawa 213-0001, Japan;Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0101, Japan;Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Science, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-0032, Japan;Kazusa DNA Research Institute, Yana 1532-3, Kisarazu, Chiba 292-0812, Japan

关键词: SigB;    Sigma factor;    RNA polymerase;    Chloroplast development;    Arabidopsis thaliana;    NEP;    nuclear gene-encoded RNA polymerase;    PEP;    plastid-encoded RNA polymerase;    PSII;    photosystem II;    PFD;    photon flux density;   

DOI  :  10.1016/S0014-5793(00)02216-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Development of plastids into chloroplasts, the organelles of photosynthesis, is triggered by light. However, little is known of the factors involved in the complex coordination of light-induced plastid gene expression, which must be directed by both nuclear and plastid genomes. We have isolated an Arabidopsis mutant, abc1, with impaired chloroplast development, which results in a pale green leaf phenotype. The mutated nuclear gene encodes a sigma factor, SigB, presumably for the eubacterial-like plastid RNA polymerase. Our results provide direct evidence that a nuclear-derived prokaryotic-like SigB protein, plays a critical role in the coordination of the two genomes for chloroplast development.

【 授权许可】

Unknown