Heat‐shock proteins during growth and sporulation of Bacillus subtilis

FEBS Letters

Todd, J.A.⁴  Travers, A.A.³  Ellar, D.J.¹  Hubbard, T.J.P.² 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QW England;Laboratory of Molecular Biology, Department of Crystallography, Birkbeck College, Malet Street, London, England;MRC Laboratory of Molecular Biology, Hills Road, Cambridge, CB2 2QH, England;Department of Medical Microbiology, Fairchild Building, Stanford University School of Medicine, Stanford, CA 94305, USA

关键词: Bacillus subtilis;    Heat-shock protein;    Sporulation;    RNA polymerase;    Sigma factor;    SpoOA;   

DOI  :  10.1016/0014-5793(85)80373-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Four major heat-shock proteins (hsps) with apparent molecular masses of 84, 69, 32 and 22 kDa were detected in exponentially growing stationary phase and sporulating cells of Bacillus subtilis heat-shocked from 30 to 43°C. The most abundant, hsp69, is probably analogous to the E. coli groEL protein. These proteins were transiently inducible by heat-shock. Partial purification of RNA polymerase revealed several other minor hsps. One of these, a 48 kDa polypeptide probably corresponds to σ⁴³. The synthesis of this polypeptide and at least two other proteins appeared to be under sporulation and heat-shock regulation and was affected by the SpoOA mutation.

【 授权许可】

Unknown