Mechanism for the peroxynitrite scavenging activity by anthocyanins

FEBS Letters

Osawa, Toshihiko²  Kato, Yoji³  Tsuda, Takanori¹ 

[1] Tokaigakuen Women's College, 2-901 Nakahira, Tenpaku-ku, Nagoya 468-8514, Japan;Nagoya University Graduate School of Bioagricultural Sciences, Nagoya 464-8601, Japan;School of Humanities for Environmental Policy and Technology, Himeji Institute of Technology, Himeji 670-0092, Japan

关键词: Anthocyanin;    Nitric oxide;    Peroxynitrite;    3-Nitrotyrosine;    Antioxidant;    C3G;    cyanidin 3-O-β-D-glucoside;    HPLC;    high-performance liquid chromatography;    LDL;    low-density lipoprotein;    MS;    mass spectrometry;    NO;    nitric oxide;    NT;    3-nitrotyrosine;    ONOO−;    peroxynitrite;    Pel;    pelargonidin;    UV;    ultraviolet;   

DOI  :  10.1016/S0014-5793(00)02150-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We show that anthocyanins can function as potent inhibitors of the formation of nitrated tyrosine in vitro, and clarified how pelargonidin (Pel), which has a mono-hydroxyl group on the B-ring, can scavenge peroxynitrite (ONOO⁻) by detection of the reaction products. Pel was reacted with ONOO⁻, then the reaction mixture was analyzed using high-performance liquid chromatography (HPLC). The HPLC analyses showed two novel peaks assumed to be the reaction products. Based on the instrumental analyses, the reaction products were identified as p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid. Pel can protect tyrosine from undergoing nitration through the formation of p-hydroxybenzoic acid and 4-hydroxy-3-nitrobenzoic acid.

【 授权许可】

Unknown