Peroxynitrite‐induced hemolysis of human erythrocytes and its inhibition by antioxidants

FEBS Letters

Kondo, Hiroshi¹  Niki, Etsuo¹  Takahashi, Mareyuki¹ 

[1]Research Centre for Advanced Science and Technology, University of Tokyo, 4-6-1 Komaba, Meguro, Tokyo 153, Japan

关键词: Nitric oxide;    Peroxynitrite;    Hemolysis;    Antioxidants;    AAPH;    2;    2′-azobis(2-amidinopropane) dihydrochloride;    NAC;    N-acetylcysteine;    NO;    nitric oxide;    PBS;    phosphate buffered saline;    PCOOH;    phosphatidylcholine hydroperoxide;    PEOOH;    phosphatidylethanolamine hydroperoxide;    SIN-1;    3-morpholinosydnonimine;   

DOI  :  10.1016/S0014-5793(97)00922-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It was found that human erythrocytes underwent hemolysis when incubated with peroxynitrite at 37°C under air. The extent of hemolysis increased with increasing peroxynitrite concentration and decreasing hematocrit. The peroxynitrite-induced hemolysis was suppressed only partially by a radical scavenging antioxidant such as uric acid and Trolox, a water-soluble vitamin E analogue, but reduced glutathione, N-acetylcysteine and albumin efficiently inhibited the hemolysis. A selenium-containing organic compound, ebselen, also suppressed the hemolysis. On the other hand, nitric oxide and superoxide generated concomitantly from 3-morpholinosydnonimine (SIN-1) did not induce appreciable hemolysis, while it converted hemoglobin to methemoglobin extensively.

【 授权许可】

Unknown