Stabilization of erythrocytes against oxidative and hypotonic stress by tannins isolated from sumac leaves ( Rhus typhina L.) and grape seeds ( Vitis vinifera L.)

Cellular & Molecular Biology Letters

Ewa Olchowik³  Maksim Ionov¹  Karol Lotkowski³  Maria Bryszewska¹  Saidmukhtar Mavlyanov²  Maria Zamaraeva³  Nodira Abdullajanova² 

[1] Department of General Biophysics, University of Łódź, Łódź, Poland$$;Institute of Bioorganic Chemistry, Academy of Sciences of Uzbekistan, Tashkent, Uzbekistan$$;Department of Biophysics, University of Białystok, Białystok, Poland$$

关键词: Tannins;    Erythrocytes;    Oxidative stress;    Peroxynitrite;    Hypochlorous acid;    Hemolysis;    Fluorescence anisotropy;   

DOI  :  10.2478/s11658-012-0014-7

学科分类：分子生物学，细胞生物学和基因

来源: Uniwersytet Wroclawski * Wydzial Biotechnologii / University of Wroclaw, Faculty of Biotechnology

【 摘 要 】

Erythrocytes are constantly exposed to ROS due to their function in the organism. High tension of oxygen, presence of hemoglobin iron and high concentration of polyunsaturated fatty acids in membrane make erythrocytes especially susceptible to oxidative stress. A comparison of the antioxidant activities of polyphenol-rich plant extracts containing hydrolysable tannins from sumac leaves (Rhus typhina L.) and condensed tannins from grape seeds (Vitis vinifera L.) showed that at the 5–50 μg/ml concentration range they reduced to the same extent hemolysis and glutathione, lipid and hemoglobin oxidation induced by erythrocyte treatment with 400 μM ONOO− or 1 mM HClO. However, extract (condensed tannins) from grape seeds in comparison with extract (hydrolysable tannins) from sumac leaves stabilized erythrocytes in hypotonic NaCl solutions weakly. Our data indicate that both hydrolysable and condensed tannins significantly decrease the fluidity of the surface of erythrocyte membranes but the effect of hydrolysable ones was more profound. In conclusion, our results indicate that extracts from sumac leaves (hydrolysable tannins) and grape seeds (condensed tannins) are very effective protectors against oxidative damage in erythrocytes.

【 授权许可】

Unknown