FEBS Letters | |
A tetanus toxin sensitive protein other than VAMP 2 is required for exocytosis in the pancreatic acinar cell | |
Padfield, Philip J1  | |
[1] Department of Pathology, Saint Louis University Medical Centre, South Grand Boulevard, St. Louis, MO 63104, USA | |
关键词: Pancreatic acinus; Botulinum neurotoxin; Tetanus toxin; Exocytosis; Secretion; | |
DOI : 10.1016/S0014-5793(00)02126-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The neurotoxin sensitivity of regulated exocytosis in the pancreatic acinar cell was investigated using streptolysin-O permeabilized pancreatic acini. Treatment of permeabilized acini with botulinum toxin B (BoNT/B) or botulinum toxin D (BoNT/D) had no detectable effect on Ca2+-dependent amylase secretion but did result in the complete cleavage of VAMP 2. In comparison, tetanus toxin (TeTx) treatment both significantly inhibited Ca2+-dependent amylase secretion and cleaved VAMP 2. These results indicate that regulated exocytosis in the pancreatic acinar cell requires a tetanus toxin sensitive protein(s) other than VAMP 2.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020309938ZK.pdf | 178KB | download |