A tetanus toxin sensitive protein other than VAMP 2 is required for exocytosis in the pancreatic acinar cell

FEBS Letters

Padfield, Philip J¹ 

[1] Department of Pathology, Saint Louis University Medical Centre, South Grand Boulevard, St. Louis, MO 63104, USA

关键词: Pancreatic acinus;    Botulinum neurotoxin;    Tetanus toxin;    Exocytosis;    Secretion;   

DOI  :  10.1016/S0014-5793(00)02126-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The neurotoxin sensitivity of regulated exocytosis in the pancreatic acinar cell was investigated using streptolysin-O permeabilized pancreatic acini. Treatment of permeabilized acini with botulinum toxin B (BoNT/B) or botulinum toxin D (BoNT/D) had no detectable effect on Ca²⁺-dependent amylase secretion but did result in the complete cleavage of VAMP 2. In comparison, tetanus toxin (TeTx) treatment both significantly inhibited Ca²⁺-dependent amylase secretion and cleaved VAMP 2. These results indicate that regulated exocytosis in the pancreatic acinar cell requires a tetanus toxin sensitive protein(s) other than VAMP 2.

【 授权许可】

Unknown