The rat hepatoma cell line H4‐II‐E‐C3 expresses high activities of the high‐affinity glutamate transporter GLT‐1A

FEBS Letters

Pollard, Matthew¹  McGivan, John¹ 

[1]Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, UK

关键词: Glutamate transporter;    Rat hepatoma;    GLT-1;    High-affinity;   

DOI  :  10.1016/S0014-5793(00)02114-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The rate of uptake of aspartate into the rat hepatoma cell line H4-II-E-C3 is very much higher than that exhibited by normal rat hepatocytes. Using an RT-PCR-based strategy, a glutamate transporter resembling mouse liver GLT-1A has been cloned from H4-II-E-C3 cells. Northern blotting confirmed that relatively high levels of mRNA for GLT-1A are expressed in hepatoma cells compared with negligible levels in rat hepatocytes. To our knowledge, this is the first report of the cloning of a high-affinity glutamate transporter from a transformed cell line and also the first demonstration of functional expression of GLT-1 outside the central nervous system.

【 授权许可】

Unknown