Glutamate‐induced calcium increase in myotubes depends on up‐regulation of a sodium‐dependent transporter

FEBS Letters

Frank, Claudio²  Rufini, Stefano¹  Fiorentini, Carla³  Giammarioli, Anna Maria³  Falzano, Loredana³ 

[1]Department of Biology, University of Rome ‘Tor Vergata’, Via della Ricerca Scientifica, 00173 Rome, Italy

[2]Department of Pharmacology, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy

[3]Department of Ultrastructures, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161 Rome, Italy

关键词: Myogenic cell;    Adenosine;    Glutamate transporter;    Calcium;    2CA;    2-chloro-adenosine;    Glu;    glutamate;    PDC;    L-trans-pyrrolidine-2;    4-dicarboxylic acid;    THA;    DL-threo-b-hydroxyaspartic acid;    EAATs;    excitatory amino acid transporters;   

DOI  :  10.1016/S0014-5793(02)03200-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report a study on the regulation by 2-chloro adenosine (2CA) of a glutamate (Glu) transporter in myogenic C2C12 cells. Long-term 2CA exposition significantly increased the V _max of the Glu transporter. Moreover, 2CA-treated cells responded to Glu challenge by a rapid and transient increase in their intracellular calcium level. The above reported effects were totally abolished by treating C2C12 cells with the Na⁺-dependent Glu transporter inhibitors DL-threo-b-hydroxyaspartic acid and L-trans-pyrrolidine-2,4-dicarboxylic acid. We propose that the possible link between the Glu uptake increase and the Glu induction of calcium rise could be the depolarizing currents carried by Na⁺ coupled with transporter activity.

【 授权许可】

Unknown