FEBS Letters | |
Inactivation of a MAPK‐like protein kinase and activation of a MBP kinase in germinating barley embryos | |
Vennik, Marco1  Testerink, Christa1  Wang, Mei1  Heimovaara-Dijkstra, Sjoukje1  Kijne, Jan W.2  | |
[1] Center for Phytotechnology, Leiden University/the Netherlands Organization for Applied Scientific Research (UL/TNO), TNO Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands;Center for Phytotechnology, UL/TNO, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands | |
关键词: Mitogen-activated protein kinase; Myelin basic protein kinase; Germination; Rehydration; Hordeum distichum; ABA; abscisic acid; MAPK; mitogen-activated protein kinase; MBP; myelin basic protein; | |
DOI : 10.1016/S0014-5793(00)02112-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos.
【 授权许可】
Unknown
【 预 览 】
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RO201912020309923ZK.pdf | 285KB | download |