Inactivation of a MAPK‐like protein kinase and activation of a MBP kinase in germinating barley embryos

FEBS Letters

Vennik, Marco¹  Testerink, Christa¹  Wang, Mei¹  Heimovaara-Dijkstra, Sjoukje¹  Kijne, Jan W.² 

[1] Center for Phytotechnology, Leiden University/the Netherlands Organization for Applied Scientific Research (UL/TNO), TNO Department of Applied Plant Sciences, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands;Center for Phytotechnology, UL/TNO, Institute of Molecular Plant Sciences, Leiden University, Wassenaarseweg 64, 2333 AL Leiden, The Netherlands

关键词: Mitogen-activated protein kinase;    Myelin basic protein kinase;    Germination;    Rehydration;    Hordeum distichum;    ABA;    abscisic acid;    MAPK;    mitogen-activated protein kinase;    MBP;    myelin basic protein;   

DOI  :  10.1016/S0014-5793(00)02112-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We provide evidence for involvement of two different 45 kDa protein kinases in rehydration and germination of barley embryos. In dry embryos, a myelin basic protein (MBP) phosphorylating kinase was detected, which could be immunoprecipitated with an anti-MAPK (mitogen-activated protein kinase) antibody. Rehydration of the embryo induced a decrease in activity of this 45 kDa MAPK-like protein kinase. In addition, activity of a MBP kinase of the same molecular weight was subsequently found to be induced. This second MBP kinase activity could not be immunoprecipitated with the anti-MAPK antibody and was induced only in germinating embryos, not in dormant embryos.

【 授权许可】

Unknown