The MAP kinase kinase NtMEK2 is involved in tobacco pollen germination

FEBS Letters

Coronado, Marı́a-José²  Testillano, Pilar S²  Voronin, Viktor³  Wilson, Cathal³  Touraev, Alisher³  Heberle-Bors, Erwin³  Risueño, Maria-Carmen²  Laurière, Christiane¹  Limmongkon, Apinun³  Barinova, Ioulia³  Aionesei, Tatiana³ 

[1] Institut des Sciences du Végétal, UPR 2355, CNRS, 1 Av. de la Terrasse, 91198 Gif sur Yvette Cedex, France;Laboratory of Plant Development and Nuclear Organization, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain;Max F. Perutz Laboratories, University Departments at the Vienna Biocenter, Institute of Microbiology and Genetics, University of Vienna, Dr. Bohr-Gasse 9, A-1030 Vienna, Austria

关键词: Mitogen-activated protein kinase;    Mitogen-activated protein kinase kinase;    Pollen;    Germination;    GFP;    green fluorescent protein;    GST;    glutathione S-transferase;    MAP kinase;    mitogen-activated protein kinase;    MBP;    myelin basic protein;   

DOI  :  10.1016/S0014-5793(04)00076-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tobacco ntf4 mitogen-activated protein (MAP) kinase gene (and its encoded protein p45^Ntf4) is expressed at later stages of pollen maturation. We have found that the highly related MAP kinase SIPK is also expressed in pollen and, like p45^Ntf4, is activated upon pollen hydration. The MAP kinase kinase NtMEK2 activates SIPK, and here we show that it can also activate p45^Ntf4. In an attempt to inhibit the function of both MAP kinases simultaneously we constructed a loss-of-function mutant version of NtMEK2, which, in transient transformation assays, led to an inhibition of germination in the transformed pollen grains. These data indicate that NtMEK2, and by inference its substrates p45^Ntf4 and/or SIPK, are involved in pollen germination.

【 授权许可】

Unknown