The MAT1 cyclin‐dependent kinase‐activating kinase (CAK) assembly/targeting factor interacts physically with the MCM7 DNA licensing factor

FEBS Letters

Xu, Fan¹  Hall, Frederick L.¹  Wang, Yan¹ 

[1] Department of Molecular Pharmacology and Toxicology, University of Southern California School of Pharmacy, Los Angeles, CA 90033, USA

关键词: Ménage à trois1;    Minichromosome maintenance 7;    Licensing factor;    Protein–protein interaction;    Yeast two-hybrid system;    Cell cycle control;    CDK;    cyclin-dependent kinase;    CAK;    cyclin-dependent kinase-activating kinase;    CTD;    carboxyl-terminal domain;    MAT1;    ménage à trois1;    MCM;    minichromosome maintenance;   

DOI  :  10.1016/S0014-5793(00)02117-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

MAT1 (ménage à trois1) functions as an assembly/targeting factor of CAK (cyclin-dependent kinase-activating kinase). In a search for MAT1-interacting proteins using yeast two-hybrid system, MCM7 (minichromosome maintenance 7), a member of a family of DNA licensing factors, was identified. The physical interaction between MAT1 and MCM7 was confirmed in vivo in yeast cells and verified with in vitro protein binding assays. Further studies showed the RING-finger motif of MAT1 is not required for the interaction with MCM7, while the C-terminal domain of MAT1 is indispensable. Immunoprecipitation of MCM7 in human osteosarcoma MG63 cells demonstrated that MCM7 associates with the CAK complex in vivo.

【 授权许可】

Unknown