Regulation of tumour necrosis factor α mRNA stability by the mitogen‐activated protein kinase p38 signalling cascade

FEBS Letters

Clark, Andrew R¹  Saklatvala, Jeremy¹  Sully, Gareth¹  Brook, Matthew¹ 

[1] Kennedy Institute of Rheumatology Division, Imperial College School of Medicine, 1 Aspenlea Road, Hammersmith, London W6 8LH, UK

关键词: Tumor necrosis factor α;    Mitogen-activated protein kinase p38;    mRNA stability;    Translation;    Macrophage;    Lipopolysaccharide;   

DOI  :  10.1016/S0014-5793(00)02084-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The translation of tumour necrosis factor α (TNFα) mRNA is regulated by the stress-activated protein kinase p38, which also controls the stability of several pro-inflammatory mRNAs. The regulation of TNFα gene expression in a mouse macrophage cell line RAW264.7 was re-examined using an inhibitor of stress-activated protein kinases. Stimulation of these cells with bacterial lipopolysaccharide resulted in stabilisation of TNFα mRNA, which was reversed by specific inhibition of p38. An adenosine/uridine-rich element from the TNFα 3′ untranslated region conferred p38-sensitive decay in a tetracycline-regulated mRNA stability assay. Therefore the p38 pathway also controls TNFα mRNA turnover.

【 授权许可】

Unknown