FEBS Letters | |
Identification of a novel 300‐kDa factor termed IκBαE3‐F1 that is required for ubiquitinylation of IκBα | |
Furuichi, Kiyoshi1  Chiba, Tomoki2  Kobayashi, Masato1  Takeuchi, Masahiro1  Tanaka, Keiji2  Suzuki, Hiroshi1  | |
[1] Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co., Ltd., 21 Miyukigaoka, Tsukuba-shi, Ibaraki 305-8585, Japan;The Tokyo Metropolitan Institute of Medical Science, and CREST, Japan Science and Technology Corporation (JST), 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113-8613, Japan | |
关键词: Ubiquitin; SCF; IκB; NF-κB; Tumor necrosis factor α; | |
DOI : 10.1016/S0014-5793(99)01173-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Destruction of IκB by ubiquitinylation is required for signal-dependent activation of NF-κB. The IκBα ubiquitin-ligase activity associated with phosphorylated IκBα (pIκBα) in HeLa cells was almost completely lost by washing under stringent conditions including 1 M NaCl; nevertheless, an SCFβTrCP complex containing Skp1, Cullin-1, and F-box/WD40 protein βTrCP was still bound to pIκBα, suggesting the existence of a putative factor that is loosely associated with pIκBα and may collaborate with SCFβTrCP. The factor was named IκBαE3-F1 and was partially purified from HeLa cells. Gel filtration analysis revealed that IκBαE3-F1 has an apparent molecular mass of approximately 300 kDa.
【 授权许可】
Unknown
【 预 览 】
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