Serine base‐exchange in rat liver nuclei

FEBS Letters

Meljon, Anna¹  Dygas, Anna¹  Barańska, Jolanta¹  Przybyłek, Krzysztof¹ 

[1] Nencki Institute of Experimental Biology, Department of Molecular and Cellular Neurobiology, 3 Pasteur Street, 02-093 Warsaw, Poland

关键词: Serine base-exchange;    Phosphatidylserine;    Nucleus;    Rat liver;    cADPr;    cyclic ADP-ribose;    ER;    endoplasmic reticulum;    HEPES;    4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid;    InsP3;    inositol 1;    4;    5-trisphosphate;    MAM;    mitochondria associated membranes;    PKC;    protein kinase C;    PS;    phosphatidylserine;    TCA;    trichloroacetic acid;   

DOI  :  10.1016/S0014-5793(00)02058-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has been shown that the incorporation of [¹⁴C]serine into phosphatidylserine (PS) in isolated rat liver nuclei is intrinsic to this organelle as attested by marker enzyme activity. Serine incorporation into PS was the highest in nuclei depleted of the outer membrane of the nuclear envelope (nucleoplasts) and negligible in the outer membrane. Trypsin treatment of nucleoplasts caused a strong inactivation of PS synthesis and only a moderate one of the NAD pyrophosphorylase activity, the marker enzyme of the inner nuclear membrane. We suggest that the serine base-exchange enzyme is located in the inner membrane of the nuclear envelope and accessible from the periplasmic surface of this membrane.

【 授权许可】

Unknown