Nuclear localization of a hypoxia‐inducible novel non‐symbiotic hemoglobin in cultured alfalfa cells1

FEBS Letters

Kovács, László²  Lukács, Noémi²  Seregélyes, Csaba²  Kiss, György B.¹  Kovács, Izabella²  Ayaydin, Ferhan²  Dudits, Dénes²  Sass, László²  Horváth, Gábor V.²  Mustárdy, László²  Vass, Imre²  Endre, Gabriella¹ 

[1] Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701 Szeged, Hungary;Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences, P.O. Box 521, 6701 Szeged, Hungary

关键词: Non-symbiotic hemoglobin;    Immunolocalization;    Hypoxia;    Cell division cycle;   

DOI  :  10.1016/S0014-5793(00)02049-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a 483-bp-long full-length cDNA clone encoding a non-symbiotic hemoglobin called Mhb1, the first one found in alfalfa. This non-symbiotic hemoglobin is a single copy gene localized in linkage group 4 in diploid Medicago genome. The Mhb1 mRNA was found only in the roots of alfalfa plants. The Mhb1 gene was inducible by hypoxia and showed no induction by cold stress treatment. The Mhb1 transcript level increased at the G2/M boundary in a synchronized alfalfa cell suspension culture. The majority of Mhb1 protein was shown to be localized in the nucleus and smaller amounts were detected in the cytoplasm. A potential link to the nitric oxide signalling pathway is also discussed.

【 授权许可】

Unknown