Maternal cold inducible RNA binding protein is required for embryonic kidney formation in Xenopus laevis

FEBS Letters

Kok, K.H.⁴  Xu, Ren-He¹  Peng, Ying⁵  Kwok, K.H.H.²  Kung, Hsiang-fu²  Fung, P.C.W.⁴  Tay, David³  Lin, Marie C.M.² 

[1] WiCell Research Institute, 614 Walnut St., Madison, WI, USA;Institute of Molecular Biology, The University of Hong Kong, 8/F Kadoorie Biological Science Bldg., Pokfulam Rd, Hong Kong, PR China;Department of Anatomy, The University of Hong Kong, Hong Kong, PR China;Division of Medical Physics, Department of Medicine, The University of Hong Kong, Hong Kong, PR China;Department of Neurology, The First Military Medical University of China, Guangzhou, PR China

关键词: Cold inducible RNA binding protein;    cDNA cloning;    Pronephros;    Morphogenetic movement;    Xenopus laevis;    CIRP;    cold inducible RNA binding protein;    RA;    retinoic acid;    Xlim-1;    LIM class homeobox containing gene;    WT1;    Wilm's tumor suppressor gene;    Xbra;    Xenopus brachyury;    EF-1α;    elongation factor-1 α;    AC;    animal cap;   

DOI  :  10.1016/S0014-5793(00)02019-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We cloned a major isoform of Xenopus homologue of cold inducible RNA binding protein (CIRP), XCIRP-1. XCIRP-1 was neither cold inducible nor essential for cell division during early embryonic development. Suppression of XCIRP-1 dose dependently produced tailbuds with deformations of the brain and internal organs. The defects were XCIRP-1 specific as they could be rescued by sense transcript. Suppression of XCIRP-1 also disrupted the morphogenetic migration of the C3 blastomeres (lineaged to become the embryonic kidney, the pronephros). In animal cap explants, depletion of XCIRP-1 inhibited activin/retinoic acid induced expressions of pronephros related Xlim-1 and WT1 genes. These results suggest that XCIRP-1 is required for the specification and morphogenetic lineage migration of the pronephros.

【 授权许可】

Unknown